Squalene (SQ), a natural precursor of many steroids, can inhibit tumor progression and decrease serum cholesterol levels.\nHowever, it is difficult to discern the effect of highly active molecules in the treatment of diseases because not enough active\ncompounds reach the site of pathology in crowded biosystems. 2erefore, it is necessary to design artificial probes that work\neffectively within crowded systems. In this study, to facilitate cell penetration, the ethylene glycol moiety (used as a probe) was\nchemically added to SQ to form 2-(2-hydroxyethoxy)-3-hydroxysqualene (HEHSQ). HEHSQ was prepared from 2,3-epoxysqualene\nand characterized by Rf, FT-IR, 1H NMR, 13C NMR, and high-resolution mass spectrometry. We then evaluated the\nanti-inflammatory effects of SQ and HEHSQ on lipopolysaccharide- (LPS-) stimulated RAW264.7 murine macrophages. To\ndetermine the effect of SQ and HEHSQ on the viability of RAW264.7 cells, an MTT assay was performed. To quantify the antiinflammatory\neffect of SQ and HEHSQ, we measured nitric oxide (NO) production, gene expression, and secretion of the\nproinflammatory cytokine tumor necrosis factor.......................
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